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VOL. 11, ISSUE 1 (2026)
Behavioural disruption of adult Culex and Anopheles mosquitoes using low-frequency acoustic stimuli: A statistically validated experimental study based on musical sargam frequencies
Authors
Dr. Arjun Shukla
Abstract
Mosquitoes exhibit a highly specialized auditory system tuned to detect
wing-beat frequencies essential for mating and orientation. Unlike humans,
mosquitoes respond to biologically relevant low-frequency vibrations via the
antennal Johnston’s organ. The present study statistically evaluates the
behavioural responses of adult Culex and Anopheles mosquitoes, including both
males and females, to controlled low-frequency acoustic stimuli derived from
musical sargam-mapped keyboard tones. A factorial experimental design was
employed, exposing mosquitoes to continuous acoustic signals at 392 Hz, 440 Hz,
and 494 Hz, along with a no-sound control. Behavioural responses were
quantified using a Behavioural Response Index (BRI) and analysed using
three-way ANOVA followed by Tukey’s post-hoc test. Results revealed a
significant main effect of frequency (p < 0.001), with 440 Hz producing the
highest behavioural disruption across both species, particularly in males.
Significant interaction effects between species, sex, and frequency were also
observed. Although no mortality occurred, sustained behavioural disruption
suggests potential applications of low-frequency acoustics in sound-assisted
mosquito management and behavioural interference strategies.
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Pages:275-277
How to cite this article:
Dr. Arjun Shukla "Behavioural disruption of adult Culex and Anopheles mosquitoes using low-frequency acoustic stimuli: A statistically validated experimental study based on musical sargam frequencies". International Journal of Entomology Research, Vol 11, Issue 1, 2026, Pages 275-277
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