The present investigation entitled ‘‘Seasonal incidence and management of Varroa destructor (Anderson and Trueman) in Apis mellifera L. Colonies of Kangra valley of Himachal Pradesh’’ carried out from July 2024 to June 2025 in the Kangra valley of Himachal Pradesh (stationary conditions) and migratory regions of Anupgarh, Rajasthan, aimed to study the seasonal incidence and management of significant ectoparasitic mite Varroa destructor in Apis mellifera colonies. Comprehensive monthly observations were recorded under both stationary and migratory setups. The infestation patterns of Varroa destructor closely followed the seasonal trends, with maximum infestation observed during June and a sharp reduction during colder months, attributed to limited brood availability and lower ambient temperatures. In Varroa destructor, the highest levels of infestation was recorded in June under both conditions, with sticky paper, brood infestation, and per hundred bee count methods indicating 46.00 mites/colony, 48.20% brood infestation, and 44.60 mites/100 bees, respectively. Evaluation of management strategies using plant-based essential oils demonstrated that Thyme oil was most effective in controlling V. destructor, reducing brood infestation from 48.00% to 5.66% across three applications. Cinnamon, Peppermint, and Rosemary oils also showed moderate efficacy. These findings highlight the significant influence of seasonal variations on mite population dynamics and validate the potential use of essential oils particularly Thyme is as effective, sustainable, and eco-friendly alternatives to conventional chemical acaricides for the management of Varroa destructor in Apis mellifera colonies.