This study was conducted in Atmida region of Dakahlia Governorate, Egypt, during the summer of season 2023, for assessing and comparing the effectiveness of a conventional pyrethroid insecticide (Deltamethrin) with 3 biorational alternatives (Emamectin benzoate, Teflubenzuron, Metarhizium anisoplia) against grasshopper nymphs of Heteracris littoralis (Rambur, 1838) (Orthoptera: Acrididae). Field results showed that the biorational insecticide emamectin benzoate was the most effective, with 85% and 99% mortality at 1 and 10 day post treatment respectively, compared to the traditional insecticide deltamethrin, which provided a rapid mortality (90% after 1 day) but slightly lower long-term effectiveness (95% after 10 days). Other biorational agents acted more slowly; teflubenzuron showed delayed activity, with toxicity increasing from 5% at 24 hours to 92% at 10 days, whereas M. anisopliae resulted in an 85% mortality rate after 10 days. All treatments significantly reduced total protein and lipid content; however, teflubenzuron caused the most severe reduction in total proteins (from 45.2 mg/g in control to 25.4 mg/g), whereas M. anisopliae and deltamethrin caused the greatest reductions in total lipids (14.1 mg/g and 15.2 mg/g, respectively, compared to 28.4 mg/g in control). Emamectin benzoate induced significantly increased on glutamic oxaloacetic (GOT) and glutamc pyruvic (GPT) transaminases activity (48.6, 65.4 U/L), followed by deltamethrin (35.2, 42.1 U/L) and M. anisopliae (28.9, 34.8 U/L) compared to control (12.5, 18.2 U/L) for GPT and GOT activity, respectively, while teflubenzuron showed the least biochemical disruption, with GPT and GOT levels. Thus, adding biorational agents into an integrated pest management (IPM) program could effectively control grasshoppers while reducing the harmful impacts of conventional pesticides.