The present study was carried out to identify the biocidal effects of hexane leaf extract of Anisomeles malabarica on Spodoptera litura under laboratory condition. Bioassays on third instar larvae were done to assess mortality, developmental abnormalities, and growth parameters. The result of the study exhibited that no larval mortality was observed; however, significant developmental disruptions occurred at later stages. Pupal malformation ranged from 11.11 to 55.56 per cent, with the highest in elution 5, while adult malformation reached 55.56 per cent in elution 2. Significant reductions in pupal weight (up to 64.15%) and length (~28%) indicated strong growth inhibitory effects. Phytochemical screening revealed the presence of flavonoids, phenols, saponins, proteins, and carbohydrates, while tannins, steroids, and terpenoids were absent. The findings suggested that hexane-soluble compounds of A. malabarica acted primarily as insect growth regulators rather than acute toxins, highlighting their potential as eco-friendly botanical agents for sustainable pest management.