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VOL. 11, ISSUE 2 (2026)
Reduced expression of acid ceramidase-1 (asah-1) alters fecundity, lifespan and lipid levels in Caenorhabditis elegans mitochondrial mutants
Authors
Nikhita Deka, Lipika Khataniar, Roshan Sarmah, Aiswarya Baruah
Abstract
In Caenorhabditis elegans, RNAi-mediated knockdown and other
genetic mutations in the mitochondrial electron transport chain (ETC) have
revealed the molecular basis of lifespan and developmental outcomes associated
with mitochondrial dysfunction. An important transcription factor, CEP-1, a
homolog of mammalian p53, regulates longevity in ETC mutants in response to
mitochondrial stress and differentially regulates a few genes depending on the
ETC mutations. Out of these, a gene, asah-1, encoding N-acylsphingosine
amidohydrolase 1, is an ortholog of human asah1, which is important in
sphingolipid and fatty acid metabolism and breaks down ceramide to sphingosine
and fatty acid. In humans, mutations in this gene are associated with diseases
such as sphingolipidosis and Farber lipogranulomatosis. Here, we show the
physiological relevance of knocking down asah-1 in C. elegans
wild-type and mitochondrial mutants, isp-1(qm150) and gas-1(fc21),
by investigating its effects on lifespan, reproduction, gene expression,
development and overall body lipid levels. This has provided new insights into
the functional relevance of asah-1 in metabolism and physiology in C.
elegans.
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Pages:146-156
How to cite this article:
Nikhita Deka, Lipika Khataniar, Roshan Sarmah, Aiswarya Baruah "Reduced expression of acid ceramidase-1 (<i>asah-1</i>) alters fecundity, lifespan and lipid levels in <i>Caenorhabditis elegans</i> mitochondrial mutants". International Journal of Entomology Research, Vol 11, Issue 2, 2026, Pages 146-156
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