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VOL. 11, ISSUE 1 (2026)
Identification and characterization of bacterial isolates associated with the abdomen of Anopheles gambiae s.l. Linneus (Diptera: Culicidae) mosquitoes
Authors
Ekerette I B, Nnodim L C, Francis P C
Abstract
Mosquitoes are recognized for their role in
the transmission of several parasitic and microbial diseases. This research was
conducted to identify and characterize the diversity of microbes inhabiting the
abdomen of Anopheles gambiae s.l. Linneus (Diptera:Culicidae)
due to their possible impact on the vector’s ability to transmit Plasmodium
species to man. Adult Anopheles gambiae s.l. Linneus
(Diptera:Culicidae) were collected from houses in Rumuepirikom Town, Rivers
State, Nigeria, identified using keys and examined for bacterial species using
standard microbiological procedures. Nine bacterial species were identified,
including Pseudomonas sp., Staphylococcus sp., Streptococcus
sp., Acinetobacter sp., Klebsiella sp., Bacillus sp., Serratia
sp., Escherichia sp., and Enterobacter sp. Of these, Pseudomonas
sp, Serratia marcescens, Staphylococcus sp.,
Acinetobacter sp., Klebsiella sp., Bacillus sp., and Enterobacter
cloacae were isolated from the blood-fed mosquitos while the non-blood
fed group harboured Staphylococcus sp., Streptococcus sp., Acinetobacter
sp., Klebsiella sp., Bacillus sp., and Escherichia coli.
Total Heterotrophic Bacteria Count (THBC) of Anopheles gambiae s.l. Linneus
(Diptera: Culicidae) sampled ranged from 6.34±0.23 to 6.68±0.10 (LogCFU/ml) and
Salmonella sp count was zero count. The bacterial count for blood fed 1,
blood fed 2, blood fed 3 and non-blood fed Anopheles gambiae s.l. Linneus
(Diptera: Culicidae) mosquito recorded are 6.34±0.23, 6.63±0.24, 6.68±0.10 and
6.46±0.17 (LogCFU/ml) with p-value > 0.05. Two one-sided tests (TOST)
analysis revealed inequivalence in the bacterial load between blood-fed and
non-blood fed. This research identified the bacterial species associated with
the abdomen of Anopheles gambiae s.l. Linneus (Diptera: Culicidae)
mosquito populations, which are mostly pathogenic gram-negative rods, and there
is disparity in the microbial load between blood fed and non-blood fed Anopheles
gambiae s.l. Linneus (Diptera: Culicidae) mosquitoes.
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Pages:175-179
How to cite this article:
Ekerette I B, Nnodim L C, Francis P C "Identification and characterization of bacterial isolates associated with the abdomen of <i>Anopheles gambiae </i>s.l.<i> </i>Linneus (Diptera: Culicidae)<i> </i>mosquitoes". International Journal of Entomology Research, Vol 11, Issue 1, 2026, Pages 175-179
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