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VOL. 10, ISSUE 9 (2025)
Isolation and identification of bacterial colonies from the gut of cockroach (Periplaneta americana), slender ant (Tetraponera spp.) and mosquito (Culicidae spp.)
Authors
Dr. T Elizabeth Thangamani Sunitha, MK Sriram, Y Abinaya, C Manisha
Abstract
Insects such as cockroaches (Periplaneta americana), slender ants
(Tetraponera spp.), and mosquitoes (Culicidae spp.) serve as vectors for a
variety of pathogenic microorganisms that can affect human health. This study
investigated the morphological identification and quantitative enumeration of
bacterial colonies isolated from the guts of these insects. Gut samples were
collected from various locations and processed according to ISO standard
protocols, which included surface sterilization, gut dissection, and
homogenization. Culturing was performed using Nutrient agar to support general
bacterial growth and MacConkey agar to isolate Gram-negative enteric bacteria
selectively. The isolated colonies were further characterized by Gram staining
and detailed examination of colony morphology. Results indicated that while all
three insect types harbored diverse bacterial communities, the mosquito gut
exhibited a particularly high bacterial load. These findings highlight the
potential of targeting gut bacteria as a biocontrol strategy to mitigate the
insects’ role in disease transmission.
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Pages:55-58
How to cite this article:
Dr. T Elizabeth Thangamani Sunitha, MK Sriram, Y Abinaya, C Manisha "Isolation and identification of bacterial colonies from the gut of cockroach (<i>Periplaneta </i><i>americana</i>), slender ant (<i>Tetraponera </i>spp<i>.)</i> and mosquito (<i>Culicidae </i>spp.)". International Journal of Entomology Research, Vol 10, Issue 9, 2025, Pages 55-58
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