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International Journal of
Entomology Research
ARCHIVES
VOL. 10, ISSUE 8 (2025)
Comparative study on the larvicidal effect of neem extract on Culex, Anopheles, and Aedes species
Authors
Santhosh Goud, Subhasmita Pattnaik, Simani Mohanty, Kiran Bala Bhuyan, Somanath Sahoo, Subasini Pattnaik
Abstract
The widespread use of synthetic insecticides in mosquito control has raised concerns regarding environmental impact, toxicity to non-target organisms, and the development of resistance among vector species. In light of these concerns, there is increasing interest in alternative, environmentally safe insecticides derived from plant sources. This study investigates the larvicidal potential of neem (Azadirachta indica) leaf extract on the fourth instar larvae of Culex quinquefasciatus, Anopheles stephensii, and Aedes aegypti. Neem extract was obtained via Soxhlet extraction using different solvents to prepare concentrations ranging from 0 to 1%. Larvicidal assays were conducted over 24, 48, and 72 hours, with mortality rates recorded at each time point. Probit analysis was applied to determine each species' LC50 and LC90 values. Results revealed significant larvicidal activity of neem extract, particularly against Aedes aegypti, which exhibited the highest mortality rates. The findings highlight neem extract as a promising eco-friendly and cost-effective alternative to synthetic insecticides for mosquito vector control.
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Pages:12-18
How to cite this article:
Santhosh Goud, Subhasmita Pattnaik, Simani Mohanty, Kiran Bala Bhuyan, Somanath Sahoo, Subasini Pattnaik "Comparative study on the larvicidal effect of neem extract on Culex, Anopheles, and Aedes species". International Journal of Entomology Research, Vol 10, Issue 8, 2025, Pages 12-18
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