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VOL. 10, ISSUE 8 (2025)
Isolation of a new trypsin inhibitor from Calopogonium Mucunoides and its toxicity to the larvae of the Dengue vector, Aedes albopictus
Authors
Deepthi M, Kannan Vadakkadath Meethal
Abstract
Plant protease inhibitors are molecules that
inhibits proteolytic activity of proteases. They regulate the activity of
proteases and also serves as defence proteins. The expression of some plant
protease inhibitors is induced by herbivory. These defence proteins can be
exploited in pest control. The predominant protease in the gut of mosquito
larvae is trypsin-like serine proteases. Thus, serine protease inhibitors find
applications in mosquito control. In this investigation, 45 plant extracts were
screened for trypsin inhibition activity. Of these, 11 extracts exhibited
trypsin inhibition greater than 50%. Six of these protease inhibitors were
proteinaceous in nature and among them Calopogonium mucunoides had shown
the highest trypsin inhibition and showed significant toxicity to Aedes
albopictus larvae. The C. mucunoides trypsin inhibitor was
purified by ammonium sulphate fractionation, gel filtration chromatography, ion
exchange chromatography and trypsin affinity chromatography. The purified
trypsin inhibitor was found to have 22.54 ± 0.27 percentage trypsin inhibition at 0.04 µg/µl protein
concentration. When Aedes albopictus
larvae were treated with fraction eluted from gel filtration column,
significant mortality observed with an LC50 (48
hours) value of 0.32 ± 0.00 µg/ml.
As the trypsin inhibitor from C. mucunoides is toxic to A. albopictus
larvae and it will find applications in mosquito control.
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Pages:25-32
How to cite this article:
Deepthi M, Kannan Vadakkadath Meethal "Isolation of a new trypsin inhibitor from <i>Calopogonium Mucunoides</i> and its toxicity to the larvae of the Dengue vector, <i>Aedes albopictus</i>". International Journal of Entomology Research, Vol 10, Issue 8, 2025, Pages 25-32
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