Plant and Insect are having unique relationship of food chain. Insects belongs to Lepidopteran shows fecundity mores based on choice methods of host plants on not only choice methods of host plants not only for egg laying but also survival of futher generation by actively feeding all life stages on host plant species. During our study we found 48 host plants among 63 noted cultivated species of botanical garden of college belongs to 19 families viz., Asteraceae, Verbenaceae, Fabaceae, Rubiaceae, Apocynaceae, Euphorbiaceae, Moringaceae. For to check diversity of butterflies Line transect method, was used. About 69 butterfly’s species were recorded among them, 20 species belong to Nymphalidae, 12 belong to Pieridae, 12 belongs to Papilionidae and 25 species belong to Lycaenidae.As preferred host plant such as Tridax procumben, Tagetes (Marigold) (Family: Asteraceae) harbored 10 species of Pieridae, 12 species of Nymphalidae as well 8 species of Lycaniedae During our study we found host plant relationship between host plant and butterfly species. A weed plant with multicolored flowers, Lantana camara (Family:Verbenaceae) hosted 6 species of Papilionidae, 3 species of Pieridae, and 9 species of Nymphalidae, similarly Euphorbia hirta (Family:Euphorbiaceae) and Tephrosia purpurea (Family: Fabaceae) hosted 9 species of Nymphalidae, and 4 Papilionidae and about 10 species from Lycaenidae found on plant Cosmos sulphurus (Family:Asteraceae).The present study thus revealed that there is need for the conservation of varied species of butterflies, which were hazardously influenced by the rising cementary and cutting of forest vegetation, thus raising Botanical gardens in college level enhances mode of conservation of diversity of not only butterflies but also helps to conserve floral diversity of botanical gardens.