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VOL. 10, ISSUE 4 (2025)
DNA barcording and molecular phylogenetic analyses of Siphlonurus mayfly species (Ephemeroptera: Siphlonuridae) in Japan
Authors
JaeIck JO, Rie Saito
Abstract
Mayflies of the genus Siphlonurus
(Ephemeroptera, Siphlonuridae) are distributed
throughout the Northern Hemisphere. However, information of species
distribution and the presence of DNA sequences in reference databases for
species identification of Siphlonurus are limited in Japan. To enhance species identification and gather DNA information
within this genus, DNA sequences from the mitochondrial cytochrome c oxidase
subunit I (COI), nuclear DNA of histone H3, and the 18S rRNA regions were analyzed.
The study focused on three species (S. binotatus, S. sanukensis, and S.
yoshinoensis), with eighteen specimens collected from eastern Japan. The phylogenetic tree of a phylogeny using
all three genes (COI + histone H3 + 18SrRNA) strongly supported monophyly at
species level. For S. yoshinoensis, we found that they have high
genetic difference within the species for COI and histone H3 regions.
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Pages:93-98
How to cite this article:
JaeIck JO, Rie Saito "DNA barcording and molecular phylogenetic analyses of <i>Siphlonurus</i> mayfly species (Ephemeroptera: Siphlonuridae) in Japan". International Journal of Entomology Research, Vol 10, Issue 4, 2025, Pages 93-98
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