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International Journal of
Entomology Research
ARCHIVES
VOL. 10, ISSUE 3 (2025)
Piper longum as grain protectant: Evaluation of its phytochemical profile, bioefficacy and repellent activity against Sitophilus species
Authors
Sowmya G, Sanjay M S, Meghashree A M, Ramyashree M
Abstract
Significant portion of the global population relies on Agriculture as their primary occupation. Especially in the country like India, over 70% of the population directly depends on agriculture for their livelihood. The loss of food grains due to insect pests during storage is a major concern in India. The application of chemical insecticides to control insects has substantial challenges andseveral drawbacksincluding the emergence of resistant strains, presence of harmful toxic residues and worries over worker safety associated with their use.To address these issues, there is an urgent need to develop environment friendly, long lasting affordable technique to protect grains from insect pests. Plant-based insecticides can therefore serve a crucial role in integrated pest management. The primary aim of the study is to analyse P.longum as grain protectant and to analyse its phytochemical properties and repellent activity. Various concentrations of ethanol and methanol extracts were tested for their insecticidal effects against Sitophilus species. The average mortality percentage of Sitophilus species indicates the toxic and repellent effect which is proportional to the concentration of the extract. Higher concentrations exhibit a more pronounced effect. Phytochemical analysis of ethanol extract confirms the presence of phytosterols and methanol extract showed the presence of alkaloids and phytosterols. The LC50 value of ethanol extract after 72 hours of exposure was 144.023mg/L and LC50 value of methanol extract after 48 hours of exposure was 89.990 mg/L. The methanol extract appears to be more effective than the ethanol extracts.The average repellence percentage of P.longum was 57.69% at 500mg/L concentration. The P-Value of ethanol and methanol extracts were 1.557 X 10-100and 2.26546 X 10-50respectively which was less than 0.01 which shows the results obtained are significant. The experimental studies demonstrated P.longum as grain protectant against Sitophilus species. Itsnatural insecticidal property and ethno pharmacological properties are the evidencesthat it does not pose any toxic hazard from the treated grain. So, P.longum can be considered as an ideal grain protectant from the point of view of seed viability and safety to mammals.
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Pages:139-146
How to cite this article:
Sowmya G, Sanjay M S, Meghashree A M, Ramyashree M "<i>Piper longum</i> as grain protectant: Evaluation of its phytochemical profile, bioefficacy and repellent activity against Sitophilus<i> </i>species". International Journal of Entomology Research, Vol 10, Issue 3, 2025, Pages 139-146
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