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VOL. 10, ISSUE 3 (2025)
Differential climbing patterns based on gender, mating status, age, and Lithium Chloride (LiCl) Treated Drosophila melanogaster
Authors
Vijaykumar Rajashekhar, Srinath Sridhar Belgaum, Nanjaiah Shivanna
Abstract
The climbing assay (negative geotaxis)
evaluates locomotor behavior in Drosophila melanogaster. The study
examines the effects of age, mating status, and lithium chloride (LiCl) treatment
on climbing ability. Flies were grouped according to gender, mating status and
different ages with varying concentrations of LiCl (15 mM, 45 mM, and 75 mM).
The climbing performance of flies was measured at different intervals of
distances ranging from 0-5 cm to 15-20 cm and the time was noted. Results
showed that younger flies exhibited better climbing ability than old ones.
Flies treated with 15 mM LiCl performed better than higher concentrations and
control. Virgin flies consistently outperformed mated ones. MANOVA confirmed
significant differences across age, groups, treatment, and distance,
highlighting the influence of both age and LiCl treatment on locomotion. These
findings provide insights into the effects of aging, mating, and chemical treatments
on locomotor performance, presenting a valuable method for studying behavioral
physiology found in different animals.
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Pages:126-132
How to cite this article:
Vijaykumar Rajashekhar, Srinath Sridhar Belgaum, Nanjaiah Shivanna "Differential climbing patterns based on gender, mating status, age, and Lithium Chloride (LiCl) Treated <i>Drosophila melanogaster</i>". International Journal of Entomology Research, Vol 10, Issue 3, 2025, Pages 126-132
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