Rice fields are wetland ecosystems with a high degree of environmental heterogeneity that harbors varied fauna. The present study was carried out in agro-ecosystem at Ernakulam district to document the biodiversity of inhabiting Staphylinidae family during three different stages of rice cultivation, namely Pre-harvesting, Harvesting and Post harvesting. A total of 10,039 Staphylinidae beetles, belonging to five subfamilies viz, Oxytelinae, Staphylininae, Paederinae, Aleocharinae, Tachyporinae were collected from the study site during a 2-year period. The subfamilies, Staphylininae, Aleocharinae, Oxytelinae, Paederinae were recorded in all three stages of rice cultivation. The collected species have been observed to follow a uniform pattern of seasonal colonisation and succession. The biodiversity of irrigated rice is of concern to both agroecologists and conservation biologists. Integrated efforts can therefore result in the creation of biodiversity-based strategies as an organising principle for the sustainable management of the agro-ecosystem.