Mosquito-borne diseases such as dengue fever, malaria, yellow fever and encephalitis cause significant mortality in human and livestock in various parts of the world. In the present work, the larvicidal activity of Lufenuron, an IGR compound evaluated using laboratory experiments and field trials in the areas in and around Tiruchirappalli. The bio efficacy of the Lufenuron formulation tested against late fourth instar larvae of different mosquito species using the bioassay recommended by WHO. The formulation of Lufenuron was applied in the field at doses of 0.02ppm, 0.002 ppm, 0.0002 ppm, 0.00002 ppm and 0.000002 ppm (g/m³) in the breeding habitats of Aedes aegypti and Culex quinquefasciatus mosquitoes.