Mosquito-borne illnesses persist as substantial global health threats, necessitating safer and eco-friendly control strategies. This study evaluates the larvicidal efficacy of green-synthesized silver nanoparticles (AgNPs) derived from the aqueous leaf extract of Alstonia scholaris against the larvae of Aedes aegypti under laboratory conditions.

AgNPs were synthesized using A. scholaris leaf extract as a reducing and stabilizing agent and characterized by UV–Vis spectroscopy, FTIR, XRD, and HR-SEM. Larvicidal bioassays were performed on third-instar larvae of A. aegypti at nanoparticle concentrations ranging from 10–200 ppm. Mortality was recorded at 24, 36, 48, and 60 hours post-exposure, and LC₅₀ and LC₉₀ values were calculated using probit analysis.