ARCHIVES
VOL. 9, ISSUE 9 (2024)
Insecticidal activity of lectin from Perinereis Cultrifera against hibiscus mealybug
Authors
P Vijayalakshmi, G Anbukani, S Harikrishnan, R Balasubramanian
Abstract
The present study dealt with the evaluation of the
insecticidal activity of lectin purified from marine polychaetes collected
from the Gadilam estuary against the mealybugs infestation in Hibiscus plants. The
collected polychaete species was identified as Perinereis cultrifera. Lectin was extracted from the polychaete and
it was designated as ‘PcL’. The extracted PcL showed the
highest hemagglutination titer of 32 HU
(hemagglutination unit) against rabbit erythrocytes. Purification of the
extracted crude lectin was done using a series of purification steps and 60% ammonium sulphate
saturation results in highest protein precipitation of 22 mg/ml. This was furtuer
purified using DEAE-Cellulose and DEAE- Sephadex A-50 column chromatography
techniquies. The specific activity was found to be increased to 890.4 U/mg with
6.3 fold purity after the purification in DEAE-cellulose column whereas; it was
1280 U/mg with 9 fold purity after the DEAE-sephadex column chromatographic
purification. The purified lectin was evaluated for its insecticidal activity
against mealybugs infestation in Hibiscus plants. The results showed excellent insecticidal activity
on a dose dependent manner with the highest insect mortality of 73.33% at 10
mg/ml of lectin concentration with the LC50 value of 6.8 mg/ml. The
field study also showed effective insecticidal activity after 3 days of
application.
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Pages:34-38
How to cite this article:
P Vijayalakshmi, G Anbukani, S Harikrishnan, R Balasubramanian "Insecticidal activity of lectin from <i>Perinereis Cultrifera</i> against hibiscus mealybug". International Journal of Entomology Research, Vol 9, Issue 9, 2024, Pages 34-38
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