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VOL. 9, ISSUE 8 (2024)
Investigation into the antimicrobial property of methanol-extracted hemolymph proteins of immunized fifth instar larvae of eri silkworm Philosamia ricini (Lepidoptera: Saturniidae)
Authors
Jakrimchi A Sangma, Bhavna P Baroowa, Prasanta Kumar Choudhury
Abstract
Fifth instar larvae of Eri silkworm, Philosamia ricini were
grouped as control (C) - injected with Phosphate Buffer Saline (PBS) and
Infected (I)- injected with a bacterial suspension of Escherichia coli
to induce innate immune responses. After stipulated
time-intervals of 24 (I-24) hours and 48 hours of injection (I-48), hemolymph
samples were collected, proceeded through methanolic extraction using
Schoofs et al., (1990) [33] method to perform Bradford assay,
Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE),
disc diffusion assay, agar-well diffusion assay, and Minimum Inhibitory
Concentration (MIC). Comparative estimates of protein concentration of the
hemolymph samples showed that infected group at 24 hr of infection (I-24) had
higher protein concentration as compared to the control group. SDS-PAGE for
molecular weight determination showed prominent bands between 17 and 20 kDa in
both control and infected groups. Disc diffusion assay of antimicrobial test
showed small zones of inhibition in all the samples when they were treated with
both gram-positive bacteria, Bacillus cereus and gram-negative bacteria,
Klebsiella pneumonia. However, for agar-well diffusion assay, the zone
of inhibitions was formed by all the samples when treated with gram positive
bacteria, B. cereus. The Minimum Inhibitory Concentration (MIC) value of
the sample C-24 was found to be 2.5 mg/mL against K pneumonia, and for
sample C-24 and I-48 were found to be 1.25 mg/ml against B. cereus.
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Pages:190-196
How to cite this article:
Jakrimchi A Sangma, Bhavna P Baroowa, Prasanta Kumar Choudhury "Investigation into the antimicrobial property of methanol-extracted hemolymph proteins of immunized fifth instar larvae of eri silkworm <i>Philosamia ricini</i> (Lepidoptera: Saturniidae)". International Journal of Entomology Research, Vol 9, Issue 8, 2024, Pages 190-196
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