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VOL. 9, ISSUE 8 (2024)
In silico screening identified scopolamine as a potent anti-termite agent against Odontotermes obesus through ROS activation and enzymatic inhibition
Authors
Kranthikumar P, Apka Nageswara Rao
Abstract
Termites, particularly Odontotermes obesus pose a significant
threat to agricultural and structural integrity worldwide. Traditional chemical
pesticides often cause resistance and cause environmental concerns. Therefore,
identifying novel, effective, and eco-friendly anti-termite agents is crucial.
This study aimed to explore scopolamine as a potential anti-termite agent
through in silico screening, focusing on reactive oxygen species (ROS)
activation and enzymatic inhibition mechanisms. In silico molecular docking was
used to predict the binding affinity of scopolamine to key termite enzymes.
superoxide dismutase (SOD) inhibition assays were conducted to assess the
ability of scopolamine to disrupt antioxidant defense. The H2DCFDA
assay was used to measure intracellular ROS levels, whereas the NADPH oxidase
assay was used to evaluate the impact of scopolamine on ROS producing enzymes.
Molecular docking results indicated a strong binding affinity of scopolamine to
Endo-1,4-β-glucanase, Exo-1,4-β-glucanase, and Endo-1,4-β-Xylanase, suggesting
significant inhibitory potential. SOD inhibition assays confirmed that scopolamine
effectively impaired the antioxidant defense mechanisms in termites. H2DCFDA
assays revealed elevated ROS levels in scopolamine-treated termites, indicating
oxidative stress. NADPH oxidase assays further support the role of scopolamine
in enhancing ROS production, contributing to cellular damage and termite
mortality. The dual mechanism of ROS activation and enzymatic inhibition by
scopolamine is a robust approach for termite control. This strategy not only
ensures higher efficacy, but also mitigates the risk of resistance development.
This study underscores the potential of scopolamine as a novel and sustainable
solution for termite management, paving the way for eco-friendly pest control
strategies.
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Pages:85-90
How to cite this article:
Kranthikumar P, Apka Nageswara Rao "In silico screening identified scopolamine as a potent anti-termite agent against <i>Odontotermes obesus</i> through ROS activation and enzymatic inhibition". International Journal of Entomology Research, Vol 9, Issue 8, 2024, Pages 85-90
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