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VOL. 9, ISSUE 8 (2024)
Microbial partnerships: Exploring gut bacteria in Eudocima moths, unravelling differential gut microbiome composition of E. materna and E. phalonia (Family: Erebidae)
Authors
Monika Rathod, Ravindra Pathre, Laxmikant Shinde, Sachin Jadhav, Yogesh Kayande
Abstract
Eudocima materna
and Eudocima phalonia is a commonly found moth species of the Erebidae
family. At the same time, collection was done at nearby areas and fruit
orchards of the Jalna district of Maharashtra. The study of gut microbiota was
done on adults (EMA) and larvae (EML) of Eudocima materna and adults of Eudocima
phalonia (EPA). The larva was reared at a laboratory that fed on Tinospora
Cordifolia locally called gulvel, both the stages of E. materna and E.
Phalonia were dissected in aseptic condition under laminar airflow, and gut
samples were collected in liquid nitrogen. After 16s rRNA sequencing, we found
that bacterial symbionts vary in both stages of the life cycle of E. materna.
In adult E. materna 49% Wittichii of bacterial species of the genus
Sphingomonas are found whereas in larva only 5% wittichii bacteria were
observed. Larva of E. materna consists of 33% Komagatae bacterial
species; on the other hand, the adult consists of 0% M. komagatae
bacteria. Adult of E. Phalonia symbiotes 61% of A. woluwensis
species of genus Arthobacter, 0% of Wittichii, and E. materna has 0% of
woluwensis This is how we have compared gut microbiome based on 16s rRNA
sequencing of gut bacteria.
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Pages:9-16
How to cite this article:
Monika Rathod, Ravindra Pathre, Laxmikant Shinde, Sachin Jadhav, Yogesh Kayande "Microbial partnerships: Exploring gut bacteria in <i>Eudocima </i>moths, unravelling differential gut microbiome composition of <i>E. materna</i> and <i>E. phalonia</i> (Family: Erebidae)". International Journal of Entomology Research, Vol 9, Issue 8, 2024, Pages 9-16
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