Logo
International Journal of
Entomology Research
Header

Search

ARCHIVES
2026 ISSUES
2025 ISSUES
2024 ISSUES
2023 ISSUES
2022 ISSUES
2021 ISSUES
2020 ISSUES
2019 ISSUES
2018 ISSUES
2017 ISSUES
2016 ISSUES
VOL. 9, ISSUE 7 (2024)
Molecular characterization and toxic evaluation of Lysinibacillus sphaericus crude extract against Tetranychus urticae and its predator Neoseiulus zaheri
Authors
Hayam Fouad Ibrahim, Marwa E Barghout, Islam M Zidan, Omaima Sharaf, Amira M Roshdy
Abstract
The present study was targeted to; i) assess the production of Lysinibacillus sphaericus crude extract (Ls CE) on different solid-state fermentation, ii) assess the acaricidal activity of Ls CE against Tetranychus urticae, iii) evaluate the prey mite mediated effects of Ls CE on the predatory mite, Neoseiulus zaheri, and iv) study the genetic diversity using ISSR marker. The bio-production of Ls CE was carried out on five medium substrates; and the most promising substrates for alkaline protease production were wheat bran and fodder yeast. The efficacy of Ls CE produced on wheat bran was investigated against eggs and females of both the prey and predatory mite species at different concentrations of 100%, 50%, 25% and 12.5%, and exposure times of 24h, 48h and 144h under laboratory conditions. Females were significantly influenced and mortality percentages increased as the Ls CE concentrations and exposure time increased. Mortality of T. urticae was 87.50 ± 2.06% and N. zaheri was 6.00 ± 1.07% at 100% concentration after 144h. Additionally, the population densities and reduction percentages of T. urticae stages were evaluated after 1, 3, 7, 10, and 16 days post- LC50 values reported to T. urticae females by Ls CE treatment under semi-field- trials. The highest decrease was observed after 3-days of indirect exposure and 7-days post direct exposure. Also, the consumption rate and fecundity of N. zaheri females were conducted through prey mite mediated effects of the same LC50 value. The consumption rate and fecundity of N. zaheri females were slightly influenced in indirect treatments. Finally, the genetic diversity of treated prey and predatory mites were resulted in 51% polymorphism in T. urticae, and 16% polymorphism in N. zaheri treatments. In conclusion, current findings recommend using Ls CE from wheat bran in biological control applications.
Download
Pages:113-122
How to cite this article:
Hayam Fouad Ibrahim, Marwa E Barghout, Islam M Zidan, Omaima Sharaf, Amira M Roshdy "Molecular characterization and toxic evaluation of <i>Lysinibacillus sphaericus</i> crude extract against <i>Tetranychus urticae </i>and its predator<i> Neoseiulus zaheri</i>". International Journal of Entomology Research, Vol 9, Issue 7, 2024, Pages 113-122
Download Author Certificate

Please enter the email address corresponding to this article submission to download your certificate.