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VOL. 9, ISSUE 7 (2024)
Authentication of DNA multiplex PCR for Indian snake species for forensic purposes
Authors
Naga Jogayya Kothakota, Satheesh Ampolu, Sheerin Bashar, Nisruti Anuja Behura, Pravallika Vataparthi
Abstract
Identification of confiscated animal samples
in forensic laboratories is nearly impossible due to processing and
degradation. Consequently, DNA-based molecular methods are optimal for
fulfilling the necessary criteria. The molecular technique needed to be robust
to function with all DNA samples from different environments. We used SWGDAM (Scientific
Working Group on DNA Analysis Methods) references to validate a new multiplex
PCR technique for forensical identification of 3 vulnerable Indian snake
species-Python morulus, Ptyas mucosus, and Naja naja. The
multiplex PCR's specificity, repeatability, stability, and sensitivity were
tested on DNA samples exposed to potentially unfavorable conditions such as
chemical treatment and environmental regimes. This validation investigation
suggests that the forensic technique will identify the above Indian snake
species.
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Pages:59-63
How to cite this article:
Naga Jogayya Kothakota, Satheesh Ampolu, Sheerin Bashar, Nisruti Anuja Behura, Pravallika Vataparthi "Authentication of DNA multiplex PCR for Indian snake species for forensic purposes". International Journal of Entomology Research, Vol 9, Issue 7, 2024, Pages 59-63
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