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VOL. 9, ISSUE 4 (2024)
Characterization and Ultrastructural Development of Nosema sp. (Microsporidia) Infecting European Honey Bee, Apis mellifera by Scanning and Transmission Electron Microscopy
Authors
Mukta Mayee Kumbhar, Suman Mishra
Abstract
Nosemosis is a deadly disease of honey bee
mainly caused by three types of Microsporidia viz. Nosema apis, Nosema ceranae and Nosema neumanni.
This microsporidian infection affects the honey bee health, leading to honey
bee colony collapse and therefore pose a is a serious threat for beekeepers in
developing countries like India. The diversity and prevalence of microsporidia
in host organisms vary according to different climatic condition. In the
present investigation, the prevalence of Nosema sp. in European honey
bee was investigated. Further, ultrastructure of different developmental stages
of Nosema sp. were characterized by light microsposcopy, Scanning
Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) that
primarily infect gut tissue of European honey bee. A total of 150 Apis
mellifera were examined, out of which 90 were found positive with Nosema
sp. infection. Therefore, the prevalence percentage of Nosema sp.
infection in European honey bee was calculated as 60%. The mean size (Length ×
Width) of Nosema sp. under scanning electron microscope was measured as
4.01±0.17 µm × 2.67±0.08 µm. The exospores of the microsporidia were found
sculptured with deeper ornamentation of ridges and furrows which is the unique
characteristics of Nosema spores previously reported in European honey
bee. The thickness of the exospore and endospore were measured as 24 nm and 162
nm respectively under TEM. Ultrastructural study of Nosema sp. showed
different developmental stages which will be helpful for the management of
nosemosis disease in European honey bee.
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Pages:146-151
How to cite this article:
Mukta Mayee Kumbhar, Suman Mishra "Characterization and Ultrastructural Development of <i>Nosema </i>sp. (Microsporidia) Infecting European Honey Bee, <i>Apis mellifera </i>by Scanning and Transmission Electron Microscopy". International Journal of Entomology Research, Vol 9, Issue 4, 2024, Pages 146-151
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