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VOL. 9, ISSUE 4 (2024)
Haematocyte count and total protein contents in haemolymph of prepual stages Bombyx mori CSR2 reared on Probiotics and Artificial dye based diet
Authors
Kebaraj R, Nazeer Mohamed P Mohideen Askar Nawas, Athmanathan Balasundaram
Abstract
Bombyx mori
CSR2 double hybrid were reared on mulberry leaves supplemented with various vital
dyes (Neutral red, Rhodamine-B, Acridine orange, Eosin yellow and Light green) on
IV and V instars larva. The effect of these particular vital dyes to the B.
mori in IV and V instar haemolymph were analysed for total haemocyte count
(THC), differential haemocyte count (DC) total protein concentration. Impact of
vital dyes along with probiotic and supplementation on total count and total
protein was analysed. seven groups showed no adverse effect. Maximum THC was
observed in group VI supplemented with probiotics and eosin yellow (IV instar –
7157 and V instar – 9052) and similar trend was observed for prohaemocyte (PR),
plasmatocyte (PLS) and granulocyte (GRS). For total protein content in heamolymph
also group VI registered maximum protein content with 7.57mg/ml in IV instar
and 8.87mg/ml in V instar larva. Thus this study clearly indicates that
application of vital dyes does not have any negative impact on heamolymph
parameters.
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Pages:46-49
How to cite this article:
Kebaraj R, Nazeer Mohamed P Mohideen Askar Nawas, Athmanathan Balasundaram "Haematocyte count and total protein contents in haemolymph of prepual stages <i>Bombyx mori</i> CSR2 reared on Probiotics and Artificial dye based diet". International Journal of Entomology Research, Vol 9, Issue 4, 2024, Pages 46-49
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