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VOL. 9, ISSUE 2 (2024)
Histological assessment of toxicity impact of various dyes on midgut of fifth instar of mulberry silkworm, bombyx mori
Authors
B Nazeer Mohamed, R Kebaraj, P Mohideen Askar Nawas, Athmanathan Balasundaram, H F Seyed Mafiya Haniff
Abstract
Histological research is a useful biomarker
for assessing the toxicity of a substance. In this study, the four stages were
supplemented with five types of vital pigments (neutral red, rhodamine-B, eosin
yellow, acridine orange and light green dyes). Vital dyes were dissolved in
distilled water, mulberry leaves were immersed in each dye and stored for a few
minutes to evaporate the water that served as the first feed for test larvae.
The larvae that received normal leaves (soaked in distilled water) acted as
controls. At the end of the test period, the middle intestine was dissected
from all groups for histological analysis. The histological evaluation of the
H-E colored slide was carried out in response to the observed coloration
conditions and to the changes at 40X magnification using the Blisco 111
Pathological Microscope and camera Sony. Based on histological observations of
the middle intestine, there was no change between the control group and the
various dye treatment groups that did not undergo pathologically significant
changes, but which were confirmed by the reconstitution of various mulberry
leaves stained with silkworms. The results of this study showed that there is
no toxic effect from various supplements of dye mulberry leaves on the
silkworm, Bombyx
mori. This knowledge is crucial in the development of new pigment molecules
capable of successfully feeding the larvae of Bombyx
mori to produce an essentially
colored silk in a variety of colors and shades.
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Pages:1-4
How to cite this article:
B Nazeer Mohamed, R Kebaraj, P Mohideen Askar Nawas, Athmanathan Balasundaram, H F Seyed Mafiya Haniff "Histological assessment of toxicity impact of various dyes on midgut of fifth instar of mulberry silkworm, <i>bombyx mori</i>". International Journal of Entomology Research, Vol 9, Issue 2, 2024, Pages 1-4
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