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VOL. 8, ISSUE 9 (2023)
Immobilization of Pseudomonas aeruginosa in alginate beads for the sustained production of mosquito larvicides against dengue vector Aedes aegypti (Diptera Culicidae)
Authors
Asifa Vijayakumar, Roja Samyuktha Vaigai Meenavan, Arthi Murugesan, Jeevarathinam Antony, Muniaraj Mayilsamy
Abstract
Mosquitoes are at the center of entomological
research because of their importance as vectors of various viral and parasitic
diseases affecting humans. They carry the causative agents of diseases such as
malaria, dengue, chikungunya, west nile fever, japanese encephalitis, and zika.
The measures to control vector mosquitoes include physical, chemical, and
biological methods. Chemical methods are hazardous to the environment as the
mosquitoes are developing resistance to the initial dosage. Biological methods
such as using Bt toxin and gambusia fishes in the breeding field are generally
used to support/complement the chemical methods. Although several species of Pseudomonas
can produce mosquito larvicide as exotoxin, its survivability is very limited
in the field due to various environmental factors. The sodium alginate
immobilization techniques have been proposed for a long to increase the field
sustainability of Pseudomonas but with limited success. The advantage of
these alginate immobilization spheres is that the Pseudomonas being trapped
inside the beads, they release the exotoxin which comes out of the permeable
membrane of the beads. At the same time, the permeable membrane allows the
nutrients to pass in. In this study, we have immobilized Pseudomonas aeruginosa
in calcium alginate beads and then it is allowed to produce the larvicidal
formulations with the continuous input of the medium and the simultaneous output
of the formulations at regular intervals. The larvicidal formulations are
bio-assayed against the Aedes aegypti larvae. The results of the
bioassay showed that the larvicides collected were efficient enough to kill the
larval mosquitoes.
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Pages:73-77
How to cite this article:
Asifa Vijayakumar, Roja Samyuktha Vaigai Meenavan, Arthi Murugesan, Jeevarathinam Antony, Muniaraj Mayilsamy "Immobilization of <i>Pseudomonas aeruginosa </i>in alginate beads for the sustained production of mosquito larvicides against dengue vector <i>Aedes aegypti </i>(Diptera Culicidae)". International Journal of Entomology Research, Vol 8, Issue 9, 2023, Pages 73-77
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