Millions of individuals contract dengue each year from the Aedes aegypti mosquito. The most effective alternative methods under the environmentally sustainable control programs comprise investigating floral biodiversity and looking into the use of safer insecticides with botanical origins. Because synthetic chemical insecticides are so often used, the dengue vector has developed resistance to them. Examining the larvicidal activity, alternation in morphology and midgut histopathology by the extracts prepared from the leaf of Tinospora cordifolia on the dengue vector Ae. aegypti. The third instar larvae were used to assess the effects of aqueous, ethanolic, and petroleum ether extracts from T. cordifolia leaves. The results recorded after 24 h of treatment were shown significant (P<0.05) larvicidal activity. The aqueous, ethanolic, and petroleum ether extract with LC50 values of 380.18, 123.02, 213.79 ppm, and LC90 values of 707.94, 275.42, 457.08 ppm, respectively. Morphological and histopathological alternation in Ae. aegypti third instar larvae by leaf extracts included, short antenna, shrunken anal papillae, narrowed respiratory tube, partially darkened larvae and disorganized epithelial cells, damaged microvilli, necrotic nuclei, free cytoplasmic mass, diffused peritrophic membrane and the damaged basal lamina, respectively. The preliminary phytochemical analysis's findings demonstrated the existence of many phytoconstituents. The histopathological effects of T. cordifolia leaf extract on the midgut of Ae. aegypti larvae were first demonstrated in this work. Researchers hoped that the study's findings would aid them in better understanding the bioactivity of the plant's extract, which might be used to get rid of Ae. aegypti.