The pomegranate fruit borer, Deudorix isocrates is a serious pest of pomegranate causes remarkable economic loss of pomegranate growers. This pest was remained untouched to study its histology and histochemistry of the various organ systems which helps in understanding of the physiology of the pest. Knowledge of these fundamental aspects of pest studies is significant in pest management. Histochemistry is one of the important aspects studied by researchers to evaluate effect of insecticides to manage their population. The present research work was done to study histochemistry of midgut of larva and adult, rectal organ of adult and secretory glands of the larva and adult with respect to characterize the neutral polysaccharides, sulphated and non sulphated acid polysaccharides using PAS and Alcian Blue activities at pH 1.5 and pH 2.5.The results revealed that comparatively there was the highest accumulation of neutral polysaccharides in the apical cytoplasm and muscle coat of the larval midgut, muscle coat of the adult midgut, intima of the rectal caecum and luminal content of adult salivary glands. In the remaining tissues of the organs under study it was appeared very weak to moderate. The intima of rectal caecum and larval mandibular glands demonstrated the highest accumulation of sulphated acid polysaccharides while outer band of salivary secretion in larva, apical cytoplasm and muscle layer of adult midgut, rectal pad and luminal contents of adult salivary gland demonstrated the highest accumulation of non sulphated acid polysaccharides.The pomegranate fruit borer, Deudorix isocrates is a serious pest of pomegranate causes remarkable economic loss of pomegranate growers. This pest was remained untouched to study its histology and histochemistry of the various organ systems which helps in understanding of the physiology of the pest. Knowledge of these fundamental aspects of pest studies is significant in pest management. Histochemistry is one of the important aspects studied by researchers to evaluate effect of insecticides to manage their population. The present research work was done to study histochemistry of midgut of larva and adult, rectal organ of adult and secretory glands of the larva and adult with respect to characterize the neutral polysaccharides, sulphated and non sulphated acid polysaccharides using PAS and Alcian Blue activities at pH 1.5 and pH 2.5.The results revealed that comparatively there was the highest accumulation of neutral polysaccharides in the apical cytoplasm and muscle coat of the larval midgut, muscle coat of the adult midgut, intima of the rectal caecum and luminal content of adult salivary glands. In the remaining tissues of the organs under study it was appeared very weak to moderate. The intima of rectal caecum and larval mandibular glands demonstrated the highest accumulation of sulphated acid polysaccharides while outer band of salivary secretion in larva, apical cytoplasm and muscle layer of adult midgut, rectal pad and luminal contents of adult salivary gland demonstrated the highest accumulation of non sulphated acid polysaccharides.