The freshwater ecology is significantly influenced by aquatic insects. The current study examines the taxonomy and species diversity of aquatic insects in the Samaguri beel in the Nagaon region of Assam, India.. Besides this, the present study also helps us to establish the species-habitat relationship concerning the water quality of the habitat. From January to June 2022, an assessment of the aquatic insect community of Samaguri beel was done. An estimated 44 species from 6 orders and 20 families made up the water insect ecosystem that was observed during the study period. Order Hemiptera with nine families and twenty-four species, followed by Coleoptera with five families and nine species, Odonata with two families and six species, Ephemeroptera with two species belonging to a single family, and order Hymenoptera with the fewest species (including 1 species from 1 family). Insects of various species depend on different physicochemical conditions of the water and have different tolerance to the water quality. The physicochemical properties of the water of the Beel were found to be of preferable quality, which is perfect for the growth of most of the aquatic organisms and it is one of the main reasons for the highly diverse aquatic insect community in this area.