The present study aimed to assess the diversity of zooplankton in the Surendranagar district of Gujarat, India, over a one-year period, from November 2021 to October 2022, in three selected sites. A total of 35 species belonging to five distinct groups, with Site 2 (Dholi Dhaja Dam) exhibiting the highest zooplankton diversity (29) and Site 1 (Falku Dam) having the lowest (16). To understand the water quality in these sites, various physico-chemical parameters such as dissolved oxygen (DO), biological oxygen demand (BOD), total dissolved solids (TDS), total suspended solids (TSS), total solids (TS), and pH were analysed. A comprehensive understanding of these parameters can provide valuable insights into the health of these ecosystems and aid in their preservation and management. The findings of this study can serve as a foundation for future research in this field, and contribute to a better understanding of the interactions between zooplankton and the water quality in freshwater ecosystems. Overall, the study highlights the importance of monitoring and studying zooplankton diversity and associated parameters in freshwater ecosystems, as this information can provide crucial insights into the conditions of these ecosystems and contribute to their proper conservation and management.