The understanding of biology and ecological significance would contribute to future use of some orders of aquatic insect organisms. In the current investigation, the maximum and minimum populations of 536 and 423 individuals, respectively, were seen in the months of November and February and 369 and 267, respectively, in the months of August and May in the years 2020 to 2021. In the years 2020 to 2021, the months of November and February saw the highest diversity indices of 1.999 and 1.978 and the lowest diversity indices of 1.925 and 1.950. The maximum species richness of 1.115 & 1.255 was observed in the month of November & May and the minimum species richness of 1.185 & 1.159 were recorded in the month of August & February in 2020 to 2021. The maximum species richness of 0.855 & 0.85 was observed in the month of November & February and the minimum species richness of 0.8384 & 1.841 were recorded in the month of August & May in 2020 to 2021. The maximum species evenness of 0.9205 & 0.9006 was observed in the month of November & February and the minimum species evenness of 0.859 & 1.8796 were recorded in the month of August & May. The diversity profile curve for the November (536) & February (423) months was lying at the top indicating higher diversity and curve for the October (483), September (435) and August (369) & March (369), April (299) and May (267) was lying at the bottom indicating lower diversity insect biodiversity at Season I and II. The histogram graph clearly showed that the curve for the months of November, February, and March was at the top, signifying higher diversity, and the curve for the months of October, September, and August was at the bottom, signifying lower diversity insect biodiversity at Seasons I and II. The most numerous species (Coleoptera, Diptera, Orthoptera, and Hemiptera) were clearly separated by the PCA study's results with positive scores on axis X (horizontal). The most numerous (Coleoptera and Diptera) species were clearly indicated (positive line) by the current findings of this PCO study on positive axis X (0.4). According to the current findings, CCA analysis was more prevalent in November, October, and February and March of every season from 2020 to March 2021. In the current studies, paired group links were used in cluster analysis to examine the grouping of the major insect groups with high similarity, which included October and November, August and September, April and May, and February and March. In the current study, aquatic insects were employed in various studies to evaluate the biological integrity of the river ecosystem.