Stingless bees or dammar bees (Hymenoptera: Apidae: Meliponini) live under cryptic conditions, playing a pivotal role by performing pollination to various flowering plant species and produce useful hive products at diversified ecosystems. Total 8129 stingless bee live colonies and 198 deserted colonies were spatial and temporal distributed at residential and non-residential areas during various seasons in 2017 to 2019 at Bangalore rural, Chikkaballapur and Kolar districts of south-eastern Karnataka. Further, 17 different sites were chosen for nesting by stingless bees, of which nine sites were found suitable at non-residential areas and remaining sites becomes usual nest sites at residential areas. The human inhabited houses, schools and temples built with walls made of mud, stone and cement, abandoned fort walls, inner surface of the well walls, unused electric devices and sheds were offered for nesting by stingless bees at residential areas. However, at non-residential areas, rocky areas, abandoned brick mounds, tree trunks and even on soil surface have used by stingless bees for nesting. Analysis of variance of seasonal distribution of stingless bee colonies during different years at various districts of residential and non-residential areas of south-eastern Karnataka revealed considerable statistical variations. Thus, present studies are crucial for future investigations to recorded role of stingless bees in this part of the state. If it kindles the local people to take up meliponiculture, it would help contribute to the improvement of local biodiversity considerably. Stingless bees not only pollinate and propagate local flora, improve biodiversity and socio-economic conditions of local people. Therefore, stingless bee’s conservation is necessitated to restore the local biodiversity and help produce hive products for human advantage in this part of the state.