Biodiversity assessment plays vital role in biodiversity conservation. This study describes the diversity and distribution of bat fauna in arid region of Uttar Pradesh. The study was carried out in seven districts of Bundelkhand from 2019 to 2021. A total of six insectivorous bats (R. hardwickii, R. microphyllum, T. nudiventris, T. perforatus, S. heathii, M. lyra) and two frugivorous bats (R. leschenaultii, P. medius) found. The distribution of T. perforatus was first time reported from Uttar Pradesh. The most abundant species was R. microphyllum, followed by R. leschenaultii, R. hardwickii, P. medius, T. perforatus, T. nudiventris, M. lyra and S. heathii. The most populous species was R. hardwickii and least populous species was M. lyra and S. heathii. Shannon diversity index of Bundelkhand regions was 1.11351 and Evenness index was 0.533549. Jaccard index of protected roost was 0.97 and unprotected roost was 1.0. The roosts of all insectivorous and R. leschenaultii found in attic and crevices of cave, rocks crevices, monuments and abandoned buildings. Bats prefer roosting in permanent structure which possesses single entrance with zero light intensity. Temperature of roost was more than 20° C and almost stable. The population of bat in protected area was higher than unprotected.