Metarhizium is an entomopathogenic fungus that is a significant biocontrol agent for agricultural crops. Initially, the Metarhizium species were identified mostly on the basis of conidial morphological traits. However, genomic profiling has made it possible to classify this fungus appropriately. This genus has 30 species, with a few species having a varied host range. To be an effective biocontrol agent, this fungus must penetrate the protein-chitin-rich insect cuticle and nourish host tissues via the secretion of a large number of degradative enzymes. Metarhizium has been used as a microbial biocontrol agent against insect pests that devastate economically significant agricultural crops, as well as for the control of human disease vectors. By identifying important genes that differentiate closely related species, genomic studies can contribute in the successful commercialization of biopesticides. Human and other animal biosafety research use cryo and intragastric experiments in mammalian models. Metarhizium offers considerable potential as a non-toxic, eco-friendly alternative to chemical pesticides in integrated pest control of crop pests.