The present study aims to study the anti-snake venom activities of the local plants, which are native to the western ghats of India. These plants were found to be used by traditional healers in Maharashtra, India to treat patients bitten by snakes. The freeze-dried snake venom powder of Daboia russelli was obtained from Hindustan Snake Park, Kolkata. The Helicteres isora and Bixa orellana was identified and authenticated by botanist. Extraction of leaves of both plant by hot extraction method and further evaluated for In vitro antivnom activity using three different types of research models, Viz. phospholipase A2 activity, procoagulant activity and Fibrinolytic activity. Plant extracts concentration 0.10 to 0.14 mg for Helicteres isora and 0.14 to 0.18mg for Bixa orellana was inhibited PLA2 dependent hemolysis, 1.5 to 1.8 mg for Helicteres isora and 1.6 to 1.9 mg for Bixa orellana of plant extracts have shown neutralizing effects in coagulant activity and 0.10 to 0.18 mg for Helicteres isora and 0.14 to 0.18 mg for Bixa orellana showed fibrinolytic activity induced by Daboia russelli venom.