This research was carried out at Sakha Agricultural Research Station, Egypt during 2020 and 2021 to investigate the genetic diversity among 14 rice genotypes for some characteristics and SSR markers linked with some specific traits. Also, the susceptibility of these genotypes to rice stem borer was assessed. Highly significant differences were found among genotypes, indicating into wide variations in genetic background. Indica/ japonica genotypes; GZ10848-1-2-2-1 and GZ10590-1-3-3-2 yielded 11.10 and11.00 t/ha., while Giza 178 and Giza 179 yielded 10.90 and 10.70 t/ha. Phenotypic coefficient of variance (PCV) was generally higher than genotypic coefficient of variance (GCV) indicating the environmental influence on the expression of traits. The highest PCV and GCV were observed in filled grains panicle-1 and panicles plant-1. The positive correlation coefficients between grain yield and number of panicles and number of filled grains/panicle indicate that better exploration of these traits could be used to develop desired rice genotypes. Dendrogram analysis divided the 14 rice genotypes into four main clusters based on the mean values of the studied traits. Out of the 14 rice genotypes, six entries were resistant or moderately resistant to stem borer, Chilo agamemnon Bles. and these entries could be candidates for developing insect-resistant varieties. The dendogram examination based on DNA level succeeded in dividing rice genotypes into two primary branches agreeing to their hereditary background. Screening, utilizing SSR markers, may be an effective instrument for varietal recognizable proof and surveying hereditary differing qualities in rice.