The existence of millions of species for living organisms and the different stages of their life ‎transformation made the classification of the ‎animal kingdom very difficult, Insects are ‎considered the most numerous in animals and their classification depends primarily on ‎‎phenotypic traits. In recent years, molecular methods have been adopted to distinguish ‎between species and genera of common descent that ‎have not been distinguished by ‎phenotypic methods. In the past ten decades, molecular methods, especially mitochondrial ‎and nuclear DNA ‎sequence data, have been used to classify insect species and genera of ‎common descent. This study aims to use the technique of random ‎polymerase chain reaction ‎with the presence of ten random primers to distinguish among Distichus planus, Harpalus ‎rufipes, Chlaenius ‎nigricornis and Brachinus bayardi, the results showed that the total ‎number of bundles is 36 and the number of polymorphic bundles is 32 ‎for all ten primers. ‎Primers OP-A04, OP-A08, OP-C06, OP-C10 and OP-C18 showed the highest percentage of ‎polymorphism which is ‎‎100%, while primer OP-C15 showed the lowest percentage of ‎polymorphism which is 60%, while primers OP-A15 and OP -B09 and OP-‎B14 No bundle ‎appeared, so the polymorphism percentage was 0%. In addition, the highest percentage of ‎primer efficiency was 25% at ‎OP-A04 and the lowest percentage was 5.55 at OP-A08. ‎Finally, the primer enabled OP-A04 was able to distinguish between the ‎Distichus planus, ‎Harpalus rufipes, Chlaenius nigricornis and Brachinus bayardi where the discriminating ‎ability was 28.125%, while the ‎primers OP-A15, OP-B09 and OP-B14 were not able to ‎distinguish between the four types because of their discriminatory ability was 0%‎.