Nosema ceranae, fungal gut parasites infect Apis mellifera. (NS) causes many problems to A. mellifera colonies and death highly infected ones. Two small-subunit rRNA regions are simultaneously amplified in a single reaction was detection of honeybee microsporidians (Nosema apis and N. ceranae). In 2019, samples from Giza region in Egypt have found in the proportions of Nosema spp. Significant spore-positive. During spring number of samples diagnosed as N. ceranae positive was found the diagnosis, while characteristic of N. ceranae a change in the tendency resulted increase in Nosema-positive samples in 2020. This is the 1STrecord of N. ceranae Egyptian government.