To resolved infestations problems of many food grains, it is now necessary to develop eco-friendly and hygienic product to preserve the food products and avoids traditional negative impact of chemical pesticides. This approach was made for formulate of biopesticides from medicinally plant, Eucalyptus globules to control infestation of food grains Vigna aconitifolius and Vigna radiates from attack of beetles, Callosobruchus maculatus. The fresh leaves of Eucalyptus globulus (nilgiri) collected, dried and extract was prepared through Soxhlet apparatus in 1:10 ratio i.e., 10 gm powder in 100 ml of different solvent systems as chloroform, acetone, and water. The mortality rate of adults released was observed after 24 and 96 hours of the exposure against different concentrations of extracts from plant, Eucalyptus globulus. Protein contents of treated (hollow) and nontreated grains were estimated by the method of [1]. The data was tabulated and result recorded. The mortality of Callosobruchus maculatus from experimental and controlled set was recorded which shown that it is directly proportional to concentration of screened biopesticides from Eucalyptus globules. It was shown that protein losses from respective grains were deterioration against concentrations of partitioned bio-pesticide. Thus, in present investigation, it was successfully formulated bio-pesticide from plant leaves of Eucalyptus globulus (nilgiri) and avoids the economical and nutritive loss of food grains.