Chemicals used to induce metabolic diseases in animal models play a crucial part in the physiology, development, and survival of the organism. For current research, Drosophila melanogaster (D.melanogaster) was used to study the toxic effects of Alloxan on mortality rate, survival, circulating metabolites, and oxidative stress. Oregon-K strain of D.melanogaster was raised in control and Alloxan treated diets and were subjected for mortality rate, survival, circulating metabolites, and antioxidative stress enzymes (ROS, SOD, and CAT). Flies treated with Alloxan showed an increased mortality rate, increased levels of glucose, trehalose, triacylglycerol, and antioxidant enzymes ROS, SOD and CAT in comparison to control flies. Further Alloxan treated flies lived significantly lesser days than the control flies. Among Alloxan-treated flies mortality rate, circulating metabolites and antioxidant enzymes increased with increasing concentration of Alloxan whereas lifespan decreased with increasing concentration of Alloxan. Sub-lethal concentration for Alloxan was noticed at 3%. Thus these results in D.melanogaster suggest that Alloxan has a toxic effect on the above traits.