Mitochondrial genome sequences as well as their comparative analysis is important from evolutionary point of view and plays a crucial role in species identification and also can be used to monitor populations. In this study comparative mitochondrial genome analysis of two fireflies which belongs to the same genus viz., Luciola cruciataand Luciola curtithorax is provided. The detailed comparative analysis of mitochondrial genome of Luciola cruciataand Luciola curtithorax provides information of mitochondrial genome organization in both species and gives overview of variation between the both species. This study provides a useful benchmark for further study on firefly mitochondrial genome evolution and their comparative analysis.