Diversity of spiders depends on prey availability and ecology of habitats. The order Araneae of Class - Arachnida includes spiders, which are important links in the terrestrial food webs and also serve as bioindicators. The present study was intended to discover the species diversity of spiders from Ahmednagar city of Maharashtra, India. The study includes collection of spiders from different sites including, Ahmednagar College campus, Bhingar suburb and Chandbibi Mahal area. The research activity was spread over a period of nine months extending from August 2019 to April 2020. 30 spider individuals belonging to 10 families were collected, out of which 23 individuals were identified upto species and 07 specimens remained unidentified. Family Araneidae was the most abundant followed by Salticidae, Thomisidae, Gnaphosidae, Lycosidae, Pholcidae, Tetragnathidae, Hersilidae, Oxyopidae and Sparassidae.