Anopheles stephensi is an important vector of urban malaria in the Indian subcontinent. The present study was planned to investigate the prevalence and distribution of this notorious vector in North Malabar region of Kerala. Larval collections were done from various potential breeding habitats, viz., domestic containers, water collection in the buildings under construction, cement tanks, wells, seepage canals, boats, fresh water ponds, streams, swamp, marshes, artificial containers, overhead tanks and ground level water tanks. The study was conducted in five North Kerala districts viz., Kasaragod, Kannur, Kozhikode, Malappuram and Wayanad from 2019 to 2021. Anopheles stephensi was found breeding in 6 breeding habitats viz., buildings under construction, cement tanks, wells, ponds, boats and ground level water tanks. The positive habitats were distributed in urban, suburban and rural areas. For the first time the species was detected from a non-coastal rural area, indicating its invasion from urban centres. Considering the efficiency of Anopheles stephensi as a vector of Malaria, constant surveillance and preventive measures are recommended.