Spirodiclofen (Envidor®), spiromesifen (Oberon®), and spiroetramat (Movento®) are an etramic acid-based insecticide, pertaining to keto-enol pesticide group, which have a novel mode of action; it intervenes with lipid biosynthesis. It's recognized as activity against the sucking insects and mites. We measured the toxicity, developmental effect and enzyme activities of this group against cotton bollworm Earias insulanaare. The result clearer that the 24 h-LC50 of spirodiclofen, spiromesifen and spirotetramat were 19.44, 16.88 and 12.05 ppm, respectively, and gave gradually decreased along three days. The compounds provided significant increased larval and pupal duration and causes malformed therefore affected on the Adult emergency and mortality of E insulanaare. Furthermore, results achieved that the use of insecticides cause significantly modification on the activities of transaminases enzymes (AST and ALT), phenoloxidase and acetylcholinesterase, total protein and lipids have highest significant decrease. Results concluded that the tetramic acid-based insecticides toxicity against Earias insulana. Later, it possible used this novel group of insecticides as integrated spiny bollworm E insulana management.