The Konkan is a shred of land on the west Indian coast of India that stretches from Thane in the north, Karwar in the south, the Arabian Sea to the west, and the Western Ghats to the east. This region is gifted with an eclectic range of floral and faunal marine biodiversity with a whopping total of 367 species of marine flora and fauna are being so far discovered from this region. Its marine biodiversity includes marine algae (73 species), mangroves (18 species), corals (11 species), molluscs (73 species), polychaetes and arthropods (47 species), sea anemones (18 species), and fishes (74 species). Not many studies are so far conducted for aquatic insects in this region. So, the goal of the present study was to understand the role of physicochemical parameters in the diversification of Aquatic insects and the role of these insects in sustaining this ecologically important zone in Maharashtra. Our study clinched seven species from four Coleoptera families and ten species from seven Hemiptera families from 8 different sites in the Konkan region. Physico-chemical characteristics were also analyzed from these different sites and their average was taken as a parameter for aquatic insect species diversity. The level of dissolved oxygen was on the low side, indicating organic contamination in the area. This research also aims to validate the impact of physicochemical conditions in aquatic insect population biodiversification.