The current examination is intended to N-Nitrosodietyhlamine induced experimental carcinogenesis: Behavioural and biochemical indices on fisetin induced vestigial mutant of drosophila melanogaster. For this study D.melanogaster used as a model organism particularyvestigial mutant ofD. melanogaster is employed as a sample organism for the present study.Mutant (vg) flies are grouped into four; they are a) control b) 0.01% NDEA treated flies c) 0.01% fisetin and 0.01% NDEAwere treated flies in food medium and group e)only 0.01% fisetin treated flies. Behavioural abnormalities like response on gravity, light, smell, taste, humidity and temperature were measured quantitatively. Dissociation between NDEA treated flies with control flies but nearly standardized in fisetin administered flies. The quantity of protein thiol, thiobarbituric acid reactive substance (TBARS), lipid peroxides and protein carbonyl also done in vg mutant fly. The biochemical compounds were notably increased in NDEA administered flies than normal flies and respectively tends to return to normal in fistien treated group. In additional, catalase (CAT), glutathione peroxidise (GPX), superoxide dismutase (SOD), reduced glutathione (GSH), and glutathione-S-transferase (GST) were reduced in cancergroup and there was sharp increase (p<0.05) in the fisetin treatment group. SEM differentiated the organs like head and intestine between NDEA treated and control vg mutant flies. Fisetin, is a bioactive phytoconstituentthat can function as a powerful antioxidant and demonstrate antiproliferative properties. Our study suggests that this may thwart abnormalities in cognitive behaviour and redox hemostasis for the duration of carcinogenesis in the vg variant of D. melanogaster.