We determined the effect of 25-azacholestane, 25-azacholesterol and N, N dimethyldodecanamine on the incorporation of [3H]-cholesterol in haemolymph, ovary, testis and fat body of Locusta migratoria. With 25-azacholestane treatment (0 to 10ppm) uptake of cholesterol by ovary decreased from 18.6% in control to 11.1% at 10 ppm and an increase was observed in fat body (26.5% to 35.8%). Similar trend was observed with 25-azacholesterol treatment. With an increase in 25-azacholesterol concentration (0 to 50 ppm), in case of female a decrease in the cholesterol uptake was observed in haemolymph (0.91% to 0.49%) and ovary (7.5% to 2.4%), while in fat body an increase from 3.3% to 5.8% was observed. In male locust also decrease in cholesterol uptake was observed in haemolymph (1% to 0.64%) but an increase is seen in testis (2.01% to 4%) and fat body (3.9% to 5.2%). However, with N, N-dimethyldodecanamine treatment, an increase in cholesterol uptake was observed both in ovary (17.6 % to 23.8%) and fat body (29.4% to 39.5%). The results were interpreted and discussed in the context of sterol metabolism inhibition as an effective strategy to control the locust population to a certain extent.