Background and objectives: Anopheles arabiensis is the main vector of malaria disease which spread all over the Sudan. This study conducted to determine the susceptibility of Anopheles arabiensis populations to some insecticides. Materials and methods: Using standard WHO diagnostic kits and procedures, the tested insecticides were DDT, fenitrothion, permethrin, deltamethrin, and bendiocarb. Results: it has been found that, An. arabiensis is fully susceptible to both deltamethrin and fenitrothion, and tolerant to each of bendiocarb and DDT. Evidence for resistance to permethrin in An. arabiensis was clearly found in two areas (Merowe –Nouri, and Eldabba) with percentage mortalities of 67.0 ±3.0%, and 65.0 ± 7.2%, respectively. Conclusion: it could be recommended that either fenitrothion or deltamethrin can be used as an alternative insecticide for indoor residual spraying campaign against An. arabiensis in the study area. Monitoring insecticide resistance in Anopheles arabiensis populations every two years is of paramount importance.