Isolation and identification of staphylococcus bacteria from fresh water fish and its antibiotics sensitivity
Sameer Ahmad Ganaie, G D Sharma
During the months of September 2018 and September 2019, a bacteriological test was carried out on 60 local healthy freshwater fish, including 30 goldfish (Carasius auratus) and 30 koi carps (Cyprinus carpio) of various weights purchased from local retail fish markets in Indore. Swabs from the skin and gills were inoculated on blood agar for 24 hours at 37 degrees Celsius (aerobic culture), and then chosen colonies were subjected to gramme staining, morphological features and biochemical assays for Staphylococcus. For all of the fish samples analysed, the proportion of Staphylococcus isolation was maximum. A total of 90 isolates from both examined fish species (48 from Carasius auratus and 42 from Cyprinus carpio) were identified, with different numbers and percentages for Isolated species were included: S. aureus (33.3%, 35.17%), S. epidermidis (25%, 28.57%), S. hyicus (22.91%, 16.66%), S. saprophyticus (12.5%, 11.90%), S. intermedius (6.25%, 7.14%) from total isolates. The results of six antibiotic sensitivity tests (Ampicillin 10 g (AM), Ofloxacin 10 g (OF), Ciprofloxacin 5 g (CIP), chloramphenicol (30 g) (CL), polymaxin-300 (Poly), and CO-Trimaxazol-25g (CO- T)) were variable. Most Staphylococcus isolates were resistant to Ampicillin but susceptible to Ciprofloxacin and Ofloxacin.
Sameer Ahmad Ganaie, G D Sharma. Isolation and identification of staphylococcus bacteria from fresh water fish and its antibiotics sensitivity. International Journal of Entomology Research, Volume 7, Issue 2, 2022, Pages 68-71