A total of 52 species of spiders belonging to 16 families and 38 genera were recorded in the Ajitmal tehsil of district Auraiya, Uttar Pradesh, India during the present study. Among them, the family Araneidae included almost one-third of the species (34.6%) followed by Salticidae (13.46%) and rest of the families comprised less than 10% of the species. Such surveys are vital for conservation of these creatures which are helpful for natural pest control and regarded as good indicators of habitat quality.